Journal: Redox Biology

Article Title: Peroxiredoxin 2 is required for the redox mediated adaptation to exercise

doi: 10.1016/j.redox.2023.102631

Figure Lengend Snippet:

Article Snippet: mouse anti-MF20 , Developmental Studies Hybridoma Bank , Cat# MF 20, RRID: AB_2147781.

Techniques: Virus, Recombinant, Protease Inhibitor, SYBR Green Assay, Membrane, Staining, Software

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 1. Development-state specific expression of basement membrane proteins in the chick embryo lens capsule. (A) Model of the lens capsule and its relationship to other compartments of the eye. The anterior capsule (red) associates with the aqueous humor and faces the cornea; the equatorial capsule (green) is directly linked to the ciliary zonules (CZ) which extend from the ciliary body (CB); and the posterior capsule (yellow) associates with the vitreous humor and faces the retina. The dia- gram represents the E15 eye, at which point the lens has matured and is the latest stage of development examined in these stud- ies. All four regions of fiber cell differentiation depicted here are present by E9. Whole embryonic chick eyes E5 (B-D), E9 (E-G), and E15 (H-J) were sectioned and stained for the three primary basement membrane proteins, laminin111 (B, E, and H, red), per- lecan (C, F, and I, green), and collagen IV (D, G, and J, purple). Overview images of the entire lens were acquired at each embry- onic stage examined, using the same settings to demonstrate the changes in expression/localization during development. Laminin111 and perlecan were present in all regions of the lens capsule at each developmental stage examined (B-I), their inten- sity lowest in the thinner anterior capsule region. While perlecan intensity and distribution appear to increase in the equatorial zone by E15, laminin localization is diminished in all regions of the capsule by this developmental time. Collagen IV developmen- tal expression and capsule distribution is distinct from laminin and perlecan, its expression limited to the posterior capsule at E5 and greatly increasing throughout all regions of the lens capsule by E15. Mag bar 50mm (B-D), 100mm (E-G) and 200mm (H-J). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Expressing, Membrane, Cell Differentiation, Staining

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 2. Dynamic basement protein remodeling in the equatorial lens capsule during development. Cryosections of embryonic chick eyes were immunolabeled for three primary basement membrane proteins, laminin111 (A, D, and G, red), perlecan (B, E, and H, green), and collagen IV (C, F, and I, purple) at E5 (A-C), E9 (D-F), and E15 (G-I). Each section was co-labeled with DAPI to detect lens equatorial epithelial cell nuclei (blue). Confocal z-stacks were acquired in the region of the equatorial lens capsule from which the images presented are a single optical plane (0.5 mm). While at E5 and E9 both laminin111 (A, D, open arrowheads) and perlecan (B,E, arrows) are distributed throughout the lens equatorial capsule, by E15 these basement membrane proteins are present in two distinct lamellae (G and H), one at the inner, lens cell-facing side of the capsule, the other at the superficial surface of the capsule where the perlecan lamella is wider than the laminin lamella. In contrast, at early stages of lens development collagen IV is primarily present only in a thin lamella along the cells of the lens equatorial epithelium (C, F, solid arrowheads) and has expanded throughout the equatorial cap- sule by E15 (I). Mag bar 20mm (A-I). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Immunolabeling, Membrane, Labeling

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 3. Bilaminar laminin/perlecan domains in the adult mouse equatorial zone. Cryosection of adult mouse eye immunolabeled for laminin111 (A,C, red) and perlecan (B,C, green), and co-labeled with DAPI to detect lens equatorial epithelial cell nuclei (blue) and imaged by confocal microscopy. The image was acquired as a single optical plane. Both laminin111 (A, arrows) and perlecan (B, arrowheads) are present as two distinct lamellae in the adult mouse lens equato- rial capsule, one at the inner, lens cell-facing side of the capsule, the other at the superficial surface of the capsule. Mag bar 20mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Immunolabeling, Labeling, Confocal Microscopy

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 4. The relationship between basement membrane proteins that comprise the anterior lens capsule. Cryo- sections of E15 chick embryo eyes were co-immunolabeled with antibodies to perlecan (green), laminin111 (teal), and collagen IV (purple), in the following combinations: (A) perlecan/laminin111, (D) collagen IV/perlecan, and (G) collagen IV/laminin111. Sections were co-labeled for F-actin (A,D,G; white) and nuclei (A-I; blue). Z-stacks were acquired of the anterior lens capsule by confocal microscopy imaging from which a single optical plane (0.5mm) is shown as an overlay of the co-immunolabeled basement membrane proteins (A,D,G), alongside of which is presented each fluorescent channel in greyscale (B,E-perlecan; C,H-laminin111; F,I-collagen IV). Collagen IV (F,I) extends throughout the anterior capsule and is detected in the anterior epithelium. Laminin (C,H) also spans this region of the lens capsule and is highly coincident with collagen IV (G). In this region of the capsule there is a perlecan gradient, its expression greatest along the inner region of the anterior capsule (E), where it is most highly coincident with collagen IV (D) and laminin (A). An additional per- lecan-rich layer is occasionally detected at the superficial surface of the anterior lens capsule (B). Mag bar 20mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Membrane, Immunolabeling, Labeling, Confocal Microscopy, Imaging, Expressing

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 5. Structural imaging of the relationship between basement membrane proteins in the anterior lens cap- sule. 3D surface structure renderings were created with Imaris software from confocal z-stacks in the region of the ante- rior lens capsule (denoted by the area within the red rectangle in the model in A) of cryosections of the E15 chick embryo eye immunolabeled for basement membrane proteins laminin (B,C,J,K), perlecan (F,G,L,M) and collagen IV (D,H). The sections were co-immunolabeled for (B-D) laminin (teal)/collagen IV (purple), (F-H) perlecan (green)/collagen IV (purple), or (J-M) laminin (teal)/perlecan (green). Each was co-labeled for F-actin (white) and nuclei (dark blue). (C,G,K,M) are zoomed in structural images of (B,F,J,L), respectively. E and I are presented to demonstrate the surface structure of the basal surface of the anterior epithelial cells shown in C and G, respectively. These structural renderings reveal that lami- nin has a typical sheet-like structure that is contacted by the basal surfaces of the lens epithelium while perlecan and col- lagen IV have a basolateral distribution, with expression of collagen IV by the lens epithelium also observed (modeled in N). Z-stack sizes (B-E) 39 slices; (F-I) 36 slices; (J-M) 36 slices. LC lens capsule. Mag bar 0.5mm (C,E,G,I,K,M), 1mm (B,D,F,H), and 3mm (J,L). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Imaging, Membrane, Software, Immunolabeling, Labeling, Expressing

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 6. The relationship between basement membrane proteins that comprise the equatorial lens capsule. Cryo- sections of E15 chick embryo eyes were co-immunolabeled with antibodies to perlecan (green), laminin111 (teal), and collagen IV (purple), in the following combinations: (A) perlecan/laminin111, (D) collagen IV/perlecan, and (G) collagen IV/laminin111. Sections were co-labeled for F-actin (D,G; white) and nuclei (A-I; blue). Z-stacks were acquired of the equatorial lens capsule by confocal microscopy imaging from which a single optical plane (0.5mm) is shown as an overlay of the co-immunolabeled basement membrane proteins (A,D,G), alongside of which is presented each fluorescent chan- nel in greyscale (B,E-perlecan; C,H-laminin111; F,I-collagen IV). In the equatorial region of the lens capsule there are two distinct laminin and perlecan lamella that are located at the inner and outer surfaces of the capsule and are highly coinci- dent (A), with perlecan (B,E) extending farther toward the center of the capsule than laminin (C,H). Collagen IV is expressed throughout the equatorial capsule (F,I), co-distributing with the perlecan/laminin lamella at the inner and outer capsule surfaces that sandwich the collagen IV-rich region between them (D,G). Collagen IV immunolabeling was most intense in a thin basement membrane zone along the capsule’s innermost surface (F,I), and at times, distinct layers of col- lagen IV were resolved across this capsule zone (F). Mag bar 20mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Membrane, Immunolabeling, Labeling, Confocal Microscopy, Imaging, Capsules

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 7. Super-resolution images of the laminin/perlecan bilateral lamella in the equatorial lens capsule. Super- resolution microscopy of cryosections of E15 chick embryo eyes co-immunolabeled for (A,C,D,F) perlecan (green) and (B,C,E,F) laminin111 (red), or immunolabeled for (G,H) nidogen (teal) imaged at the lens equatorial capsule. D,E,F,H are zoomed in regions of A,B,C,G, respectively. Images are a 0.21 mm optical slice from a confocal z-stack. There is signifi- cant co-localization of the laminin and perlecan lamella at the inner and outer surfaces of the lens (C,F). In contrast, nido- gen spans the equatorial capsule region (G,H). Mag bar: (A,B,C,G) 20mm; (D,E,F,H) 10mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Super-Resolution Microscopy, Immunolabeling

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 9. Structural imaging of the relationship between basement membrane proteins in the equatorial lens cap- sule. 3D surface structure renderings were created with Imaris software from confocal z-stacks in the region of the equa- torial lens capsule (denoted by the area within the red rectangle in the model in A) of cryosections of the E15 chick embryo eye immunolabeled for basement membrane proteins perlecan (B,C,H, green), laminin111 (D,E,I, teal) and colla- gen IV (F, purple). The sections were immunolabeled for (B,C) perlecan (green), or represent sections co-immunolabeled for (D-G) laminin (teal)/collagen IV (purple), or (H,I) perlecan (green)/laminin (teal). (B-G) were co-labeled for F-actin (white) and nuclei (dark blue), with G presented to show the structure of the basal surface of the equatorial epithelial cells depicted in E. (C,E) are zoomed in structural images of (B,D), respectively. The structural images of laminin and perlecan provide the first look of the organization of the bilateral localization of the inner and outer laminin/perlecan basement mem- brane lamellae at the inner and outer surfaces of the lens equatorial capsule (modeled in J). While collagen IV is present throughout the entire width of the equatorial lens capsule (F), the structural organization of collagen IV at the inner and outer domains of this capsular zone parallel that of laminin (D,E). Z-stack sizes (D-G) 44 slices; (B,C) 58 slices; (H,I) 54 slices. LC- lens capsule. Mag bar 0.7mm (E, G-I), 0.8mm (C), and 5mm (B, D, F). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Imaging, Membrane, Software, Immunolabeling, Labeling

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 10. The relationship between basement membrane proteins that comprise the posterior lens capsule. Cry- osections of E15 chick embryo eyes were co-immunolabeled with antibodies to perlecan (green), laminin111 (teal), and collagen IV (purple), in the following combinations: (A) perlecan/laminin111, (D) collagen IV/perlecan, and (G) collagen IV/laminin111. Sections were co-labeled for F-actin (D,G; white). Z-stacks were acquired of the posterior lens capsule by confocal microscopy imaging from which a single optical plane (0.5mm) is shown as an overlay of the co-immunolabeled basement membrane proteins (A,D,G), alongside of which is presented each fluorescent channel in greyscale (B,E-perle- can; C,H-laminin111; F,I-collagen IV). Perlecan is present as two distinct lamellae in the posterior capsule (B,E), overlap- ping with both laminin (A) and collagen IV (D), both basement membrane proteins that span this capsule zone (G-I). Mag bar 20mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Membrane, Immunolabeling, Labeling, Confocal Microscopy, Imaging

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 11. Structural imaging of the relationship between basement membrane proteins in the posterior lens cap- sule. 3D surface structure renderings were created with Imaris software from confocal z-stacks in the region of the poste- rior lens capsule (denoted by the area within the red rectangle in the model in A) of cryosections of the E15 chick embryo eye immunolabeled for basement membrane proteins laminin (B,C,H,I), perlecan (D,E,J,K), and collagen IV (F). The sec- tions were immunolabeled for (B,C) laminin, or co-immunolabeled for (D-G) perlecan (green)/collagen IV (purple), or (H- K) laminin (teal)/perlecan (green). (B-G) were co-labeled for F-actin (white), with G presented to show the structure of the basal surface of the lens fiber cells depicted in E. (C,E,I,K) are zoomed in structural images of (B,D,H,J), respectively. These structural renderings show that the bilateral organization of perlecan continues into the posterior region of the lens capsule, a wide outer lamella and a thin inner lamella (D,E,J,K), both of which are located within the laminin (H,I) and col- lagen IV (F) domains that span the posterior capsule (modeled in L). Z-stack sizes (B,C) 39 slices; (D-G) 44 slices; (H- K) 47 slices. LC lens capsule. Mag bar 1mm (C, E, G, I, and K) and 3mm (B, D, F, H, and J). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Imaging, Membrane, Software, Immunolabeling, Labeling

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 16. Fibrillin-2 extracapsular matrix is closely associated with the lens basement membrane capsule. Cryo- sections of E15 chick embryo eyes were co-labeled for (A,C,E) fibrillin-2 (green), laminin (red) and nuclei (blue), or (B,D, F) fibrillin-2 (green) and perlecan (red). Images are a single optical plane (0.5 mm) of confocal z-stacks acquired in (A,B) the upper equatorial zone at its border with the lens anterior, (C,D) at the lens equator (EQ), and (E,F) in the lens posterior region. The extracapsular fibrillin-2 matrix zone is closely associated with laminin and perlecan along the superficial layer of the lens basement membrane capsule (arrowheads). Arrows point to regions labeling for the basement membrane pro- teins alone, with the arrow on the right in C indicating the very close apposition of fibrillin-2 with the outer laminin base- ment membrane lamella. CB ciliary body, CZ ciliary zonules. Mag bar 20mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Membrane, Labeling

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

doi: 10.1016/j.matbio.2020.12.005

Figure Lengend Snippet: Fig. 17. Super-resolution images of the laminin/fibrillin-2 and perlecan/fibrillin-2 lamellae in the equatorial lens capsule. Super-resolution microscopy of cryosections of E15 chick embryo eyes co-immunolabeled for (A-D) fibrillin-2 (green) and either (A,B) laminin111 (red), or (C,D) perlecan (red) and imaged in the upper region of the lens equatorial zone near its border with the anterior of the lens (A,C) or directly at the lens equator (B,D). Each image is a 0.21 mm opti- cal slice. Fibrillin-2 is closely associated with the superficial surface of the outer laminin/perlecan lamella of the lens cap- sule. Mag bar 10 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies used in these studies are presented in Table 1 and include: Fibrillin-2 (JB3, Developmental Studies Hybridoma Bank (DSHB)), Tenascin-C (AB19013, Millipore), Fibronectin (PA1 23693, Thermofisher), Nidogen/ Entactin (1G12, DSHB), Perlecan (SC-33707, Santa Cruz), Laminin (L9393, Sigma Aldrich, Enhanced Validation), Laminin (31 or 31 2, DSHB), and Collagen IV (ab6586, Abcam).

Techniques: Super-Resolution Microscopy, Immunolabeling

Journal: eLife

Article Title: Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair

doi: 10.7554/eLife.68603

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-KRT8 (Rat monoclonal) , DSHB (TROMA-I) , RRID: AB_531826 , IF: 1:200.

Techniques: Plasmid Preparation, RNAscope, Biomarker Discovery, Software, TUNEL Assay, Staining